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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a vital function in endorsing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two extra splice variants had been claimed but detailed nucleotide sequence data is not nonetheless obtainable.
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Blocking peptides are peptides that bind especially towards the concentrate on antibody and block antibody binding. These peptide typically is made up of the epitope regarded from the antibody.
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This guide outlines even further achievable optimization of mobile and immunoassay parameters to make sure the absolute best final results are...
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This guide outlines further possible optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope on the focus on protein. By evaluating the staining from your blocked antibody as opposed to the antibody alone, you can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs into the hugely conserved cyclin family, whose members are characterised by a dramatic periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which add into the temporal coordination of each and every mitotic event. Cyclin E forms a complex with and functions for a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells development through S period.
The measurement of protein phosphorylation is a useful tool for measuring click here the modulation of receptor activation by both of those antibodies...
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